Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_O04
(763 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P40492|YIJ7_YEAST Hypothetical 59.9 kDa protein in SGA1-... 42 0.002
sp|Q96G75|U2508_HUMAN Protein UNQ2508/PRO5996 32 1.4
sp|Q91YQ7|U2508_MOUSE Protein UNQ2508/PRO5996 homolog 32 1.9
sp|P22611|VMT8_MYXVL Kelch repeat protein M-T8 31 3.2
sp|Q96TP5|ACEA_COCIM Isocitrate lyase (Isocitrase) (Isocitr... 31 3.2
sp|Q86ZF1|ACEA_LEPMC Isocitrate lyase (Isocitrase) (Isocitr... 30 5.4
sp|P29551|EF3_PNECA Elongation factor 3 (EF-3) 30 7.1
sp|Q12508|RMD5_YEAST Sporulation protein RMD5 (Glucose-indu... 30 9.3
sp|Q9PL60|Y248_CHLMU Hypothetical protein TC0248 30 9.3
>sp|P40492|YIJ7_YEAST Hypothetical 59.9 kDa protein in SGA1-KTR7 intergenic region
Length = 516
Score = 42.0 bits (97), Expect = 0.002
Identities = 40/166 (24%), Positives = 57/166 (34%), Gaps = 48/166 (28%)
Frame = +2
Query: 308 DEDWIVQRFHSFILQFYSFY--HEPSVMSLMIKTGIAVRKTENCYKPE------------ 445
D+ W V F+ FYS Y + + + + GI+ KT +C P
Sbjct: 353 DQRWSVLN-DLFLSDFYSMYGISQNDPLLIYLSLGISSLKTRDCLHPSDDENGNQETETA 411
Query: 446 -----------------MKRKECVVCIDRVNKISVDIPAARHDHSILICWKTGLEMNEDN 574
+KRK C VC + I+ +P A H S L
Sbjct: 412 TTAEKEVEDLQLFTLHSLKRKNCPVCSETFKPITQALPFAHHIQSQLF-----------E 460
Query: 575 PPIAFPNGYVYSTKAIPEL-----------------IDPTDNKVVC 661
PI PNG VY +K + +L +DP D K+ C
Sbjct: 461 NPILLPNGNVYDSKKLKKLAKTLKKQNLISLNPGQIMDPVDMKIFC 506
>sp|Q96G75|U2508_HUMAN Protein UNQ2508/PRO5996
Length = 393
Score = 32.3 bits (72), Expect = 1.4
Identities = 23/106 (21%), Positives = 50/106 (47%), Gaps = 10/106 (9%)
Frame = +2
Query: 377 SVMSLMIKTGIAVRKTENCY--------KPEMKRKECVVCIDRVNKISVDIPAARHD--H 526
S++ L +++ ++V C K +++++C + +++ ++I H
Sbjct: 274 SLLGLSVESPLSVSFASGCVALPVLMNIKAVIEQRQCTGVWNHKDELPIEIELGMKCWYH 333
Query: 527 SILICWKTGLEMNEDNPPIAFPNGYVYSTKAIPELIDPTDNKVVCP 664
S+ C + ++ NPPI G+V S A+ +LI+ K+ CP
Sbjct: 334 SVFACPILRQQTSDSNPPIKLICGHVISRDALNKLIN--GGKLKCP 377
>sp|Q91YQ7|U2508_MOUSE Protein UNQ2508/PRO5996 homolog
Length = 393
Score = 32.0 bits (71), Expect = 1.9
Identities = 23/106 (21%), Positives = 49/106 (46%), Gaps = 10/106 (9%)
Frame = +2
Query: 377 SVMSLMIKTGIAVRKTENCY--------KPEMKRKECVVCIDRVNKISVDIPAARHD--H 526
S++ L +++ ++V C K +++++C +++ ++I H
Sbjct: 274 SLLGLSVESPLSVSFASGCVALPVLMNIKAVIEQRQCTGVWSHKDELPIEIELGMKCWYH 333
Query: 527 SILICWKTGLEMNEDNPPIAFPNGYVYSTKAIPELIDPTDNKVVCP 664
S+ C + ++ NPPI G+V S A+ +LI+ K+ CP
Sbjct: 334 SVFACPILRQQTSDSNPPIKLICGHVISRDALNKLIN--GGKLKCP 377
>sp|P22611|VMT8_MYXVL Kelch repeat protein M-T8
Length = 515
Score = 31.2 bits (69), Expect = 3.2
Identities = 16/45 (35%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
Frame = -1
Query: 208 HLRVAKRMSK-FYSLFPITFFYQQIELEDLQITFKCVFDVSLFIF 77
H V SK FY+LF F + +++ DL+ +K VFDV +++
Sbjct: 30 HRLVLSAYSKYFYNLFNGNFLEKNVDVIDLEADYKTVFDVIYYMY 74
>sp|Q96TP5|ACEA_COCIM Isocitrate lyase (Isocitrase) (Isocitratase) (ICL)
Length = 538
Score = 31.2 bits (69), Expect = 3.2
Identities = 17/69 (24%), Positives = 35/69 (50%), Gaps = 12/69 (17%)
Frame = +2
Query: 47 VEQWLQSIQFKY------------KKGDVKNTFESDLQIFKLYLLIKEGDWEQAIKFAHS 190
V+QW + +++Y K+G++K + S++Q KL+ L++E + F +
Sbjct: 19 VKQWWKDSRWRYTKRPFTAEQIVAKRGNLKIEYPSNVQSKKLWKLVEEKFKTKTASFTYG 78
Query: 191 FCDPKMIKE 217
DP M+ +
Sbjct: 79 CLDPTMVTQ 87
>sp|Q86ZF1|ACEA_LEPMC Isocitrate lyase (Isocitrase) (Isocitratase) (ICL)
Length = 537
Score = 30.4 bits (67), Expect = 5.4
Identities = 18/83 (21%), Positives = 39/83 (46%), Gaps = 12/83 (14%)
Frame = +2
Query: 5 LDIVEALKQGNFHPVEQWLQSIQFKY------------KKGDVKNTFESDLQIFKLYLLI 148
+D +A Q V+QW +++Y K+G++K T+ S+ Q KL+ ++
Sbjct: 4 MDAEDAQFQKEVAEVKQWWNDSRWRYTKRTFTAEEIVSKRGNLKITYPSNSQSKKLWNIV 63
Query: 149 KEGDWEQAIKFAHSFCDPKMIKE 217
+ + + + + DP M+ +
Sbjct: 64 EHRFKNKDVSYTYGCLDPVMVTQ 86
>sp|P29551|EF3_PNECA Elongation factor 3 (EF-3)
Length = 1042
Score = 30.0 bits (66), Expect = 7.1
Identities = 15/53 (28%), Positives = 29/53 (54%), Gaps = 4/53 (7%)
Frame = -1
Query: 256 HQSHNLAQLIHRVL-FDHLRVAK---RMSKFYSLFPITFFYQQIELEDLQITF 110
H S L ++ ++ ++H ++ K MSKF +L P YQ I + +++ +F
Sbjct: 599 HDSKFLDNVVQAIIHYEHFKLKKYMGNMSKFITLVPSARSYQDISMSEIEFSF 651
>sp|Q12508|RMD5_YEAST Sporulation protein RMD5 (Glucose-induced degradation protein 2)
Length = 421
Score = 29.6 bits (65), Expect = 9.3
Identities = 19/66 (28%), Positives = 25/66 (37%)
Frame = +2
Query: 518 HDHSILICWKTGLEMNEDNPPIAFPNGYVYSTKAIPELIDPTDNKVVCPRSKCRCKESEL 697
H H I IC E +NPP + ++ S KA+ L CP S
Sbjct: 354 HFHPIFICPVLKEETTTENPPYSLACHHIISKKALDRLSKNGTITFKCPYCPVNTSMSST 413
Query: 698 QKVYIV 715
+KV V
Sbjct: 414 KKVRFV 419
>sp|Q9PL60|Y248_CHLMU Hypothetical protein TC0248
Length = 601
Score = 29.6 bits (65), Expect = 9.3
Identities = 21/66 (31%), Positives = 31/66 (46%), Gaps = 9/66 (13%)
Frame = +2
Query: 476 DRVNKISVDIPAARH----DHSILICWKTG-LEMNEDNPPIAF----PNGYVYSTKAIPE 628
D + +D+ AA + H +L CWK G +E++ P F P+ V TK I
Sbjct: 424 DNMEGYPIDLQAAEYLKSFAHQVLACWKNGDIELSTPIPLFGFEKIHPHPRVQYTKPICV 483
Query: 629 LIDPTD 646
LI+ D
Sbjct: 484 LINEQD 489
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,103,630
Number of Sequences: 369166
Number of extensions: 1723469
Number of successful extensions: 4556
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 4398
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4556
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7018522000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)